Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater of the Zainoel Abidin Hospital, Banda Aceh, Indonesia

Abstract. Anelia N, Suhartono S, Hayati Z. 2023. Abundance and phenotypic-genotypic analysis of antibiotic-resistant Escherichia coli isolated from wastewater the Zainoel Abidin Hospital, Banda Aceh, Indonesia. Biodiversitas 24: 2294-2301. Excessive inappropriate use antibiotics can initiate emergence antibiotic resistance in bacteria. Wastewater Treatment Plant (WWTP) is believed to be a reservoir for bacteria, including coli. This study aimed perform enumeration, isolation, characterization, evaluation properties resistance-coding genes encoded by plasmid E. isolates WWTP dr. General Hospital (RSUDZA). The was conducted through several stages, isolation quantification coli, testing, confirmation testing ESBL-E. isolates, DNA, amplification TEM, SHV, CTX-M genes. results showed that sum pit tank, aeration final tank contained at 7.5x104 CFU/mL, 3x102 1x102 respectively. A total 22 were obtained, with ten seven five tank. had eight types compared WWTP. Therefore, 59% classified as Multi-drug Resistant (MDR) 32% Extended Spectrum ?-Lactamase (ESBL-EC). detected all locations 63.6%, 22.7%, 95.5%, In addition, most common gene found RSUDZA hospital treatment process must improved reduce presence bacteria spread